Tag

Part:BBa_K2586006:Design

Designed by: Janek Meißner   Group: iGEM18_Goettingen   (2018-09-13)


Strep-Tag-aroA: aroA linked to a Strep-Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1387
    Illegal EcoRI site found at 74
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 74
    Illegal NheI site found at 24
    Illegal SpeI site found at 1388
    Illegal PstI site found at 1402
    Illegal NotI site found at 7
    Illegal NotI site found at 1395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 74
    Illegal BamHI site found at 95
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1388
    Illegal EcoRI site found at 74
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 74
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1388
    Illegal PstI site found at 1402
    Illegal AgeI site found at 448
    Illegal AgeI site found at 889
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 60
    Illegal BsaI.rc site found at 1123


Design Notes

This part used (BBa_K2586007) as source.

Source

We added the Strep-Tag to (BBa_K2586007) to study protein-protein interactions of the EPSP-Synthase, which is encoded by the aroA gene.

References

(Herzberg et. al. 2007) : SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics. 7:4032-4035